Biotechnological bases of design technology "green article" for control of characteristics of bioceramic protective layer of eggs shell of hens in technological process of incubation

Keywords: biotechnology, technology, chitosan, nanotechnologies, egg incubation, disinfectants.

Abstract

The authors theoretically substantiated and practically proved the effectiveness of the developed method of pre-incubation treatment of eggs using biomimetic technology "artificial cuticle" "ARTICLE" ("ARTIficial cutiCLE") "GREEN ARTICLE" to improve the structural and physiological characteristics of the shell as a bioceramic shell. in the formation on the surface of the egg of a protective film of a mixture of artificial and natural materials, which regulates the level of gas permeability of the bird's egg shell. The protective film is a multicomponent coating to restore and enhance the barrier properties of the bioceramic structures of the shell and shell membranes, which has biocidal (antibacterial and antiviral) activity, as well as the ability to optimize embryo gas exchange during incubation and improve fertilization processes. The aim of our work was to develop the technology of pre-incubation treatment of chicken eggs "GREEN ARTICLE" using protective nanocomposite coatings "Artificial Cuticle", which were created on a biomimetic basis, to reduce contamination with pathogenic microflora and increase hatchability of eggs. For the experiment, three batches of eggs were formed, which were obtained from laying hens Legorn white, 540 pieces in each group. Each batch had a control and an experimental group. Incubation was performed in an incubator "Universal" for 21 days according to the method. The first group of eggs before laying for incubation was treated with acid-soluble chitosan, the second - water-soluble chitosan, the third - water-soluble chitosan succinate. The percentage of lack of incubation, hatching and hatching of chickens was calculated. Before laying for incubation, as well as on the seventh and eighteenth day of incubation, before transferring the eggs to the hatch, took washings from the surface of the shell and examined them for microbial contamination. It is proved that the use for pre-incubation disinfection of chicken eggs of a composition based on acid-soluble chitosan with the addition of peracetic acid, hydrogen peroxide (H2O2) and metal nanoparticles (nanodisperse titanium dioxide TiO2, iron oxide Fe2O3 and copper sulfateCuSO4), stimulates the development of by 7,7% relative to control. GREEN ARTICLE technology for pre-incubation treatment of eggs using the drug "Artificial Cuticle" leads to a significant reduction in the number of pathogenic microorganisms on the surface of the shell, which leads to increased hatchability of eggs.

References

1. Bessarabov, B. F., 2006. Inkubatsiya yaits s osnovami embriologi selskohozyaystvennoy ptitsyi [Incubation of eggs with the basics of poultry embryology]. M: Kolos, pp. 264.
2. Bordunova, O. H. 2008. Biometrychna tekh nolohiiazakhystu inkubatsiinykhyaiets kurei z vykorystanniam nanokompozytiv khitozanu i dioksyduty titana [Biometric technology for protection of hatching eggs of chickens using nanocomposites of chitosan and titanium dioxide]. Tavriiskyinaukovyivisnyk. Kherson. Ailant, issue. 5, pp. 104-115.
3. Bordunova, O. H., 2010. Nanokompozyt khitozanu i dioksynu tytanu u biomimetychni i tekhnolohii zakhystu inkubatsiinykh yaiets silskohospodarskoi ptytsi [Nanocomposite of chitosan and titanium dioxin in biomimetic technology of protection of hatching eggs of poultry]. Mizhvidomchyi tematychnyi naukovyi zbirnyk Ptakhivnytstvo. Birky ,issue.65, pp. 116-127.
4. Breslavets, V. O., 2006. Doslidzhennia hazo- ta volohopronyknosti shkaralupy yaiets kurei riznykh porid ta viku [Investigation of gas and moisture permeability of eggshells of chickens of different breeds and ages]. Ptakhivnytstvo : mizhvid. temat. nauk. zb. IP UAAN, issue 58, pp. 355-360.
5. Breslavets, V. O., 2001. Inkubatsiia yaiets silskohospodarskoi ptytsi : metodychnyi posibnyk [Incubation of poultry eggs: a guide]. Kh: IEiKVM. pp. 92.
6. Medvedev, A., 2001. Bezopasnyie sredstva dlya dezinfektsii [Safe disinfectants]. Ptitsevodstvo, issue 4. pp. 37-41.
7. Prokudina, N. A., 2008. Inkubatsiya yaits selskohozyaystvennoy ptitsyi [Incubation of poultry eggs]. H.: «NTMT», pp. 386.
8. Sakhatskyi, I., 2005. Dezinfektsiini zasoby dlia ptakhivnytstva: porivnialna efektyvnist [Disinfectants for poultry: comparative effectiveness]. Veterynarna medytsyna Ukrainy, issue 1, pp. 40-43.
9. Stehnii, B. T., 2005. Porivnialna otsinka preparative dlia peredinkubatsiinoi obrobky yaiets [Comparative evaluation of drugsforpre-incubation treatment of eggs]. Mizhnarodnyi tematychnyi naukovyi zbirnyk. Kharkiv. T. 2, issue 85, pp. 1022-1025.
10. Iakubchak, O. M., 2006. Chym krashche obrobyty? Porivnialna otsinka suchasny khitradytsii nykhde zinfektsiin ykhzasobiv, shcho vykorystovuiutsia v haluzi ptakhivnytstva [What is more beautiful to grind? Ratio assessment of the occasional and traditional disinfectious diseases, how to get sick in the branch poultry]. Suchasne ptakhivnytstvo, issue 6. pp. 14-15.
11. Wei Xiao, Junbo Xu and Xiaoyan Liu., 2013. Antibacterial hybrid materials fabricated by nanocoating of microfibril bundles of cellulose substance with titania / chitosan / silver-nanoparticle composite films. J. Mater. Chem. B., issue 1, pp. 3477 3485.
12. Balaz, M., 2014. Eggshell membrane biomaterial as a platform for applications in materials science. Acta Biomaterialia. V. 10, issue 9, pp. 3827-3843. Doi :10.1016/j.actbio.2014.03.020.
13. Bain, M. M., Mcdade, K. and Burchmore, R., 2013. Enhancing the egg's natural defence against bacterial penetration by increasing cuticle deposition. Animal Genetics. V 44, issue 6, pp. 661-668. Doi: 10.1111/age.12071.
14. Bordunova, O. G., 2020. Study of the correlations between the dynamics of thermal destruction and the morphological parameters of biogenic calcites by the method of thermoprogrammed desorption mass spectrometry (TPD-MS) / Bordunova, O. G. Samokhina Y. A., Loboda, V. B., Chernenko, O M., Dolbanosova, R. V., Chivanov, V. D. // Springer Proceedings in Physics, Springer, Singapore, issue 240. pp. 37-50. https://doi.org/10.1007/978-981-15-1742-6
15. D'Alba, L., Jones, D. N. and Badawy, H. T., 2014. Antimicrobial properties of a nanostructured egg shell from a compost-nestingbird. Journal of Experimental Biology, issue 217(7), pp. 1116-1121.
16. D’Alba, L., Maia, R. and Hauber, M. E., 2016 Evolution of avian eggshell structure in relation to nesting ecology. Proc. R. Soc. Lond. B. V. 283: 0687. doi: 10.1098/rspb.2016.0687.
17. Gole, V. C., Roberts, J. R. and Sexton, M., 2014. Effect of egg washing and correlation between cuticle and egg penetration by various Salmonella strains. International Journal of Food Microbiology, issue. 182–183, pp. 18-25. doi: j. ijfoodmicro.2014.04.030.
18. Gang, Xiao., 2015. Synthesis of core-shell bioaffinity chitosan-TiO2 composite and its tnvironmental applications. Journal of Hazardous Materials, issue 283, pp. 888-896.
19. Su, Hyun Kim, Hong, Kyoon, No and Witoon Prinyawiwatkul., 2007. Effect of Molecular weight, type of chitosan, and chitosan solution pH on the shelf-life and quality of coated eggs, Journal of food science. Vol. 72, issue 1, pp. 44-48.
20. Bain, M. M., Mcdade, K. and Burchmore, R., 2013 Enhancing the egg’s natural defence against bacterial penetration by increasing cuticle deposition. Animal Genetics.DOI: 10.1111. - age. 12071.
21. Liu, Z., Sun, X. and Cai, C., 2016. Characteristics of glycos aminoglycans in chicken eggshells and the influence of disaccharide composition on eggshell properties. Poultry Science. V. 95, issue 12, pp. 2879–2888. doi: 10.3382/ ps /pew179.
22. Maria, P., Montero Garcia, M. and Carmen, G., V., 2017. Edible films and coatings: fundamentals and applications.CRC Press, Taylor & Francis Group. pp. 598.
23. Мaureen, B., Yves, N. and Filip, V., 2011. Immerseel food science, technology and nutrition improving the safety and quality of eggs and egg products: Volume 2: Egg safety and nutritional quality. Woodhead Publishing. pp. 448.
24. Yuceer, M., Caner, C., 2014 Antimicrobial lysozyme-chitosan coatings affect unctional properties and shelf life of chicken eggs during storage. J. Sci. Food Agric, issue 94, pp.153–162. doi: 10.1002/jsfa.6322.
25. Yu, Shao, Changsheng, Cao and Shiliang, Chen, 2015. Investigation of nitrogen doped and carbon species decorated TiO2 with enhanced visible light photocatalytic activity by using chitosan. Applied Catalysis B: Environmental, issue 179, pp. 344–351.
Published
2020-11-30
How to Cite
Bordunova , O., Samokhina , E., Khmelnychyi , L., Povod , M., Vechorka , V., & Popsuy , V. (2020). Biotechnological bases of design technology "green article" for control of characteristics of bioceramic protective layer of eggs shell of hens in technological process of incubation. Bulletin of Sumy National Agrarian University. The Series: Livestock, (3 (42), 27-32. https://doi.org/10.32845/bsnau.lvst.2020.3.5

Most read articles by the same author(s)

<< < 1 2 3 > >>