Cord blood serum combined with Actovegin by correction of the reproductive function of cows
Abstract
Studies have proven the role of cord blood serum and Actovegin in the recovery of postmorbid endometrium due to the content of reproductive hormones, cytokines, proenzymes, enzymes, receptors, adaptogens, growth factors, immunoregulatory agents, transport proteins and other multipotent. The above-mentioned effect from the use of cryopreserved cord blood serum is achieved during its long-term storage: -18-20◦C, as low temperature ensures the preservation of biologically active compounds in the native state and physiological ratios. The effect of cord blood serum and Actovegin on the process of remodulation of morpho-functional properties of the endometrium in the postmorbid state due to neurotrophic effects, modulation and correction of neoangiogenesis and inhibition of endometrial tissues was studied. Correction of the reproductive function of cattle will remain a problem and is of great practical and scientific interest. With high genetic potential, the reproductive capacity of cows will remain low. As a result, the level of dairy productivity decreases, the term of economic use of animals is reduced, and the profitability of the industry as a whole deteriorates. Scientists claim that reproductive performance is mainly due to environmental factors and has a low level of inheritance, so the methods of correction and stimulation are based on a thorough study of the physiological capabilities of the reproductive capacity of cows. For breeding cows, tethered and untied restraint systems are used. Leaning is characterized by the fact that cows rest and feed in stalls on a leash. Each animal has an individual feeder and drinker. Cows are also milked in stalls on a leash or in milking parlors. With tethered keeping, it is possible to carefully regulate feeding, milk cows, observe the manifestation of hunting and health, care for a particular animal, taking into account individual characteristics.
CCM and Actovegin have a biostimulating effect because they contain trace elements and biologically active components that affect the catalytic center of all known neuropeptides that are necessary for the synthesis of cellular peptides. Under the influence of Actovegin, cells of different origins increase glucose consumption and accelerate the utilization of oxygen, which activates the intracellular energy processes required during the structural and morphological rearrangement of the postmorbid endometrium. Also "Actovegin" affects the recovery of the capillary network of damaged tissue, activates erythropoiesis and erythrocyte transport function. It is proved that the use of CC stem cells is not limited to the restoration of the hematopoietic system, the body's response is unpredictable: it is possible to eliminate the pathology of nerve cells, restore the liver parenchyma and pancreatic cells, normalize homeostasis and others. Mesenchymal stem cells under the conditions of cultivation in a special way, are transformed into fibroblasts, cells of bone, adipose and fibrous tissue. The advantages of using QC include a reduced risk of transmission of some latent infections, no post-transfusion reactions and unlimited long-term storage in the frozen state. The results showed that the number of days from the correction of reproductive function to the manifestation of the stage of excitation at 10 ml was significantly lower by 14.4% (p <0.001) compared to the group of animals where the drugs were not administered. serum QC with actovegin (3.86 ± 0.36 and 14.38 ± 2.1, respectively). The number of calving cows after application of 10 ml. serum QC w / w with actovegin 10ml (400 mg) v / m (93.75%) was higher by 53.3% compared with animals where spontaneous hunting was expected. Prospects for research in this area.
The prospect of further research is to develop selected methods for correcting the reproductive capacity of cows.
Conclusions:. The number of days from correction to the manifestation of the stage of excitation was significantly lower by 14.37% (p <0.001) with the combined use of serum QC and the drug "Actovegin", compared with the same indicator where the drugs were not administered (3.86 ± 0, 36 and 14.38 ± 2.1, respectively).
The insemination index in the group of cows where the drugs were not administered was significantly higher, by 72.1% (p <0.001) relative to the group where the serum QC was used in combination with the drug "Actovegin" (1.2 and 4.3, respectively).
Thus, the use of cord blood serum and the combined use of CC serum and the drug "Actovegin" allows to restore the morphological structure of the endometrium and restore the balance between activator factors and inhibitors of angiogenesis, which determines the optimal conditions for the formation of the maternal placenta.
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